RESUMO
PURPOSE OF THE RESEARCH: We describe two interventions to screen for SARS-CoV-2 in two squats of exiled persons in France following the diagnosis of symptomatic COVID-19 cases. PRINCIPAL RESULTS: In squat A, 50 (25%) persons were screened; 19 were found positive, and three accepted a transfer. In squat B, 65 (54%) persons were screened at three different times, and only two were found positive. MAJOR CONCLUSIONS: Discrepant outcomes may reflect different levels of sanitation, prevention, and acceptance of interventions. Refusal to be transferred to specific COVID-19 homes if tested positive underscores the importance of local sanitary solutions for all. Cross-curricular strategies addressed to exiled persons are essential means of providing medical and public health solutions designed to deter COVID-19 outbreaks in these populations.
Assuntos
COVID-19/diagnóstico , Programas de Rastreamento , Migrantes , Adolescente , Adulto , França , Humanos , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto JovemRESUMO
INTRODUCTION: Haemophilia A (HA) and haemophilia B (HB) are X-linked recessive diseases, caused by a large number of pathogenic variants in the F8 and F9 genes. With the exception of introns 22 and 1 inversions which are frequent in severe HA cases, about 2000 unique variants in F8 and 1000 in F9 have been described in databases and their recurrence remains limited. AIM AND METHODS: During routine analysis, we identified two recurrent missense variants, the F8 gene c.1244C>T, p.Ala415Val variant in 27 HA patients and the F9 gene c.835G>A, p.Ala279Thr variant in 34 HB patients, in two groups of haemophiliac patients from two different regions of France. We aimed to identify whether these variants result from a founder effect. We performed haplotype reconstruction after analysis of extragenic and intragenic polymorphic markers. The ESTIAGE programme was used to estimate the age of the variant. RESULTS: We identified a common ancestral haplotype HA1, in all the HA patients sharing the p.Ala415Val variant, and HB1 for 22 of 34 HB patients sharing the p.Ala279Thr variant. The estimated time of occurrence of the founder variant was between the 13th and 17th century (95% CI: 16 to 29 generations) for the F8 variant and between the 3rd and the 11th century for the F9 variant (95% CI: 44 to 72 generations). CONCLUSION: This study supports a founder effect for these two variants in the two largest reported cohorts of haemophilia patients with an identical variant. These pathogenic variants are among the three most early reported variants in haemophilia.
Assuntos
Fator IX/genética , Fator VIII/genética , Efeito Fundador , Hemofilia A/genética , Hemofilia B/genética , Polimorfismo Genético , Estudos de Coortes , Feminino , França , Humanos , Masculino , Mutação de Sentido IncorretoRESUMO
1. A small protein of M(r) 10 kDa has been isolated by reverse-phase chromatography of the basic proteins contained in the coelomic fluid and cell lysate of the earthworm Eisenia fetida andrei. 2. The protein crossreacted in dot-blot with an anti-bovine ubiquitin antiserum. 3. Its N-terminal primary structure was determined by automatic Edman degradation on 26 consecutive amino acids and showed 69% (based on the 26 amino acids) or 82% (based on the first 19 consecutive amino acids) identity with many ubiquitins and similar charge and hydrophobicity profiles and secondary structure conformation.
Assuntos
Líquidos Corporais/metabolismo , Oligoquetos/química , Ubiquitinas/imunologia , Sequência de Aminoácidos , Animais , Reações Cruzadas , Dados de Sequência Molecular , Peso Molecular , Oligoquetos/citologia , Homologia de Sequência de Aminoácidos , Ubiquitinas/química , Ubiquitinas/isolamento & purificaçãoRESUMO
1. After bacterial infestation lysozyme and antibacterial activities are enhanced, peaking at 4 hr and 3 days, respectively. 2. Both humoral defenses require RNA and protein de novo synthesis in response to pathogenic bacteria injection (actinomycin D and cycloheximide experiments). 3. Antibacterial activity exists naturally at some basic level, involving regular translation of stable RNAs. 4. When antibacterial activity reaches its maximum after bacterial injection, proteins responsible for it undergo a turn-over. 5. Lysozyme and antibacterial proteins cannot account for the whole response to bacterial infestation; some cellular defense mechanisms like phagocytosis are involved at the same time.
